Wavefront‐sensorless adaptive optics with a laser‐free spinning disk confocal microscope

Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics

Most current superresolution (SR) microscope techniques surpass the diffraction limit at the expense of temporal resolution, compromising their applications to live-cell imaging. Here we describe a new SR fluorescence microscope based on confocal microscope optics, which we name the spinning disk superresolution microscope (SDSRM). Theoretically, the SDSRM is equivalent to a structured illumina...

Adaptive aberration correction in a confocal microscope.

The main advantage of confocal microscopes over their conventional counterparts is their ability to optically "section" thick specimens; the thin image slices thus obtained can be used to reconstruct three-dimensional images, a capability which is particularly useful in biological applications. However, it is well known that the resolution and optical sectioning ability can be severely degraded...

Simple method to improve image contrast in spinning disk microscopy using adaptive optics

Sub-Airy Confocal Adaptive Optics Scanning Ophthalmoscopy

Purpose To demonstrate the viability of improving transverse image resolution in reflectance scanning adaptive optics ophthalmoscopy using sub-Airy disk confocal detection. Methods The foveal cone mosaic was imaged in five human subjects free of known eye disease using two custom adaptive optics scanning light ophthalmoscopes (AOSLOs) in reflectance with 7.75 and 4.30 mm pupil diameters. Conf...

Adaptive Optics Confocal Scanning Laser Ophthalmoscope

Scanning laser ophthalmoscope (SLO) was first presented by R. H. Webb (R. H. Webb, et al, 1987) in 1987, which is the same as a scanning laser microscope except that human eye is used as the objective lens and retina is usually the sample being imaged. With its high contrast real-time imaging and axial sectioning capability, SLO has many advantages and applications in retina imaging (Austin. Ro...